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Additional resources for Basic Malaria Microscopy, Second Edition: Part I. Learner's Guide
40 Learning Unit 6. The microscope Oculars fitted to binocular microscopes are called paired oculars. The marking ‘x10P’ on the rim of a x10 ocular indicates that it is one of a paired set of eyepieces. 10. Arm or limb The arm forms a rigid support for the main tube and stage of the microscope. It is robust and can be used as a handle for carrying the microscope. When carrying a microscope in this way, always support the base of the microscope with the other hand. 11 and 12. Coarse and ﬁne adjustments The two adjustment systems, coarse and fine, are used to focus on the specimen being examined.
Blood in Giemsa-stained thin ﬁlms Thin films examined with the x100 oil immersion objective and the x10 ocular contain: red blood cells (or erythrocytes), white blood cells (or leukocytes) and platelets (or thrombocytes). Red blood cells The shape of the red blood cell, or erythrocyte, is described as a biconcave disc. It is the commonest cell in thin blood films. There are about 5 000 000 in each microlitre (μl) of blood. 5 μm in diameter. Sfe cmppe dfmm ps fszuispdzuf The red cell does not have a nucleus.
10. 2–32 chromatin dots and pigment? Yes: This is a schizont stage. 11. a rounded or a banana shape? Rounded: Go to 12. Banana-shaped: Go to 14. 12. clear-red chromatin and deep-blue cytoplasm in the rounded body? Yes: This is a female gametocyte. No: Go to 13. 13. that the rounded body has stained a reddish colour so that the chromatin is not clear? Yes: This is a male gametocyte. It is difﬁcult to tell the difference between male and female gametocytes in thick ﬁlms. 14. a banana-shaped parasite with densely stained blue cytoplasm and bright red chromatin?
Basic Malaria Microscopy, Second Edition: Part I. Learner's Guide by UNAIDS